Thr202/tyr204
WebFunction. ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates. Interaction. ERK1/2 phosphorylates a wide range of downstream ... WebDetect and quantitate cellular phospho-ERK1/2 (Thr202/Tyr204) in a multiplexing assay using homogeneous Alpha no-wash technology. We are now two standalone …
Thr202/tyr204
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WebERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] detects ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. WebOverview. This cell lysate provides a way to validate the Advanced phospho-ERK (Thr202/Tyr204) assay. As a positive control, it will enable researchers to avoid any detection issues when running the assay. The lysate was …
WebJan 27, 2024 · Nonspecific binding sites were blocked by SuperBlock ® blocking buffer (Thermo Fisher Scientific, Waltham, MA, USA) at RT for 1 h, and membranes were incubated overnight with anti-phospho-cPLA 2 (Ser505), anti-cPLA 2, anti-phospho-ERK (Thr202/Tyr204), or anti-total-ERK antibodies diluted to a concentration of 1:1000 with … WebErk1/2-Thr202/Tyr204磷酸化位点兔单抗(20G11克隆)#4376R, 是Apoptosis类化合物, 标准纯度52.0 μg/ml
WebMEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 … WebFor flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Clone 6B8B69 was found to strongly …
WebERK1/2 (Phospho-Thr202/Tyr204) Antibody ¥2250. TGF Beta 1+2+3 Polyclonal Antibody Cy7 Conjugated ...
WebPhospho-ERK1/ERK2 (Thr202, Tyr204) Antibody (MA5-15173) in IHC (P) Immunohistochemistry was performed on paraffin-embedded human colon carcinoma tissue. To expose target proteins, antigen retrieval was performed by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10 minutes. rice bran malt syrupWebEstimated sensitivity: Phospho-ERK (Thr202/Tyr204): 0.1 ng/mL (tested with recombinant protein), Total ERK1/2: 0.6 ng/mL (tested with recombinant protein) Range: Phospho-ERK (Thr202/Tyr204): 0.2-20ng/mL, Total ERK1/2: 0.6-60 ng/mL. Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain … rice bran meaning in teluguWeb28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. rice bran meaning in marathiWebFunction. ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates. Interaction. ERK1/2 phosphorylates a wide range of downstream ... rice bran millWebThe specific signal modulates positively in proportion to Advanced phospho-ERK1/2 (Thr202/ Tyr204). The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . culture plate. Lysates are then transferred to the assay plate for the detection of Advanced phospho-ERK1/2 (Thr202/Tyr204) by HTRF ® red hot crossbow caseWebERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates. ERK1/2 phosphorylates a wide range of downstream substrates, such as … red hot crystal clods klondikerice bran meal for horses